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ISBN-10: 4894442876

ISBN-13: 9784894442870

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The same is true for larval, fetal or adult systems. Pretreatment of objects for microvascular corrosion castings and subsequent scanning electron microscopical evaluation in generally comprises the application of a) anticoagulants, b) vasoactive drugs (vasodilatory, vasoconstrictory), or c) spasmolytic drugs. 1. Anticoagulants To receive excellent microvascular casts the system to be cast has to be free of blood which would prevent the resin fully filling the system. The administration of heparin prior to the rinsing of the circulatory system together with the addition of heparin to rinsing solutions (15000 IV/liter; 37) minimizes blood clotting.

A topic not yet discussed at all is the interrelationship between endothelial derived relaxing (EDRF) and/or contracting factors (EDCF) and the casting procedure, especially the influence of the casting media upon these factors and the resulting appearance of the cast vessel. 9. 4. Anesthesia For vascular corrosion casting animals have to be anesthetized. Besides the general conditions anesthesia has to meet according to the Good Laboratory Practice (GLP), it particularly has to assist subsequent casting.

A divergent view exists concerning the appropriate perfusion pressure of vessels. While nowadays it is generally accepted that in histological sections fixed by immersion solely, especially large elastic arteries "collapse", as indicated by the very wavy internal and external elastic membranes, and 33 Microvascular Corrosion Casting for SEM Studies measurements done on luminal diameters underestimate, there consistent view concerning the correct injecting pressure. 2. Perfusates In a critical review Hollweg and Buss [60] addressed the problems of the preparation of blood vessels for scanning electron microscopy.

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